|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human Galectin-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10290-ACG|
|Human Galectin-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10290-ACR|
|Human Galectin-1 ORF mammalian expression plasmid, C-Flag tag||HG10290-CF|
|Human Galectin-1 ORF mammalian expression plasmid, C-His tag||HG10290-CH|
|Human Galectin-1 ORF mammalian expression plasmid, C-Myc tag||HG10290-CM|
|Human Galectin-1 ORF mammalian expression plasmid, C-HA tag||HG10290-CY|
|Human Galectin-1 Gene cDNA clone plasmid||HG10290-M|
|Human Galectin-1 ORF mammalian expression plasmid, N-Flag tag||HG10290-NF|
|Human Galectin-1 ORF mammalian expression plasmid, N-His tag||HG10290-NH|
|Human Galectin-1 ORF mammalian expression plasmid, N-Myc tag||HG10290-NM|
|Human Galectin-1 ORF mammalian expression plasmid, N-HA tag||HG10290-NY|
|Human Galectin-1 natural ORF mammalian expression plasmid||HG10290-UT|
|Learn more about expression Vectors|
Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.