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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human MMP-12 ORF mammalian expression plasmid, C-GFPSpark tag||HG10266-ACG|
|Human MMP-12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10266-ACR|
|Human MMP-12 ORF mammalian expression plasmid, C-Flag tag||HG10266-CF|
|Human MMP-12 ORF mammalian expression plasmid, C-His tag||HG10266-CH|
|Human MMP-12 ORF mammalian expression plasmid, C-Myc tag||HG10266-CM|
|Human MMP-12 ORF mammalian expression plasmid, C-HA tag||HG10266-CY|
|Human MMP-12 ORF mammalian expression plasmid, N-Flag tag||HG10266-NF|
|Human MMP-12 ORF mammalian expression plasmid, N-His tag||HG10266-NH|
|Human MMP-12 ORF mammalian expression plasmid, N-Myc tag||HG10266-NM|
|Human MMP-12 ORF mammalian expression plasmid, N-HA tag||HG10266-NY|
|Human MMP-12 natural ORF mammalian expression plasmid||HG10266-UT|
|Learn more about expression Vectors|
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Macrophage metalloelastase, also known as Matrix metalloproteinase-12, Macrophage elastase, MMP12, and MMP-12, is a secreted protein which belongs to the peptidase M10A family. MMP12 is a macrophage-secreted elastase that is highly induced in the liver and lung in response to S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme responsible for cleavage of plasminogen to angiotensin, which has an angiostatic effect. It may be involved in tissue injury and remodeling and has significant elastolytic activity. It may be related to prognosis in breast cancer patients. MMP12 promotes fibrosis by limiting the expression of specific ECM-degrading MMPs. Like MMP12, MMP13 expression is highly dependent on IL-13 and type I I-IL-4 receptor signaling. MMP12 is a potent proinflammatory and oncogenic molecule. MMP12 up-regulation plays a critical role in emphysema to lung cancer transition that is facilitated by inflammation.