|Datasheet||Specific References||Reviews||Related Products||Protocols|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human FGF9 ORF mammalian expression plasmid, C-GFPSpark tag||HG10262-ACG|
|Human FGF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10262-ACR|
|Human FGF9 ORF mammalian expression plasmid, C-Flag tag||HG10262-CF|
|Human FGF9 ORF mammalian expression plasmid, C-His tag||HG10262-CH|
|Human FGF9 ORF mammalian expression plasmid, C-Myc tag||HG10262-CM|
|Human FGF9 ORF mammalian expression plasmid, C-HA tag||HG10262-CY|
|Human FGF9 natural ORF mammalian expression plasmid||HG10262-M-N|
|Human FGF9 ORF mammalian expression plasmid, N-Flag tag||HG10262-NF|
|Human FGF9 ORF mammalian expression plasmid, N-His tag||HG10262-NH|
|Human FGF9 ORF mammalian expression plasmid, N-Myc tag||HG10262-NM|
|Human FGF9 ORF mammalian expression plasmid, N-HA tag||HG10262-NY|
|Human FGF9 natural ORF mammalian expression plasmid||HG10262-UT|
|Learn more about expression Vectors|
Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.