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Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone)

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CLEC6AcDNA Clone Product Information
Gene_bank_ref_id:NM_001007033.1
cDNA Size:630
cDNA Description:ORF Clone of Homo sapiens C-type lectin domain family 6, member A DNA.
Gene Synonym:dectin-2, CLEC6A, CLECSF10
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10250-ACG$325
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10250-ACR$325
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10250-CF$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10250-CH$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10250-CM$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10250-CY$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10250-M-F$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10250-M-N$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10250-NF$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10250-NH$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10250-NM$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10250-NY$295
Human CLEC6A / CLEC4N / Dectin-2 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10250-UT$295
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Background

C-type lectin domain family 4 member N (CLEC4N), also known as Dectin-2, is a C-type lectin expressed by dendritic cells (DCs) and macrophages. Members of the C-type lectin domain (CTLD) superfamily are metazoan proteins functionally important in glycoprotein metabolism, mechanisms of multicellular integration and immunity. They share a common fold and are involved in a variety of functions, such as generalized defense mechanisms against foreign agents, discrimination between healthy and pathogen-infected cells, and endocytosis and blood coagulation. Genome-level studies on human, elegans and melanogaster demonstrated almost complete divergence among invertebrate and mammalian families of CTLD-containing proteins (CTLDcps). The vertebrate CTLDcp family was essentially formed early in vertebrate evolution and is completely different from the invertebrate families. The composition of the CTLDcp superfamily in fish and mammals suggests that large scale duplication events played an important role in the evolution of vertebrates. Dectin-2 is important in host defense against C. albicans by inducing Th17 cell differentiation. Dectin-2 constitutes a major fungal pattern recognition receptor (PRR) that can couple to the Syk-CARD9 innate signaling pathway to activate DCs and regulate adaptive immune responses to fungal infection.

References
  • Robinson MJ, et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection. J Exp Med. 206(9): 2037-51.
  • Saijo S, et al. (2010) Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans. Immunity. 32(5): 681-91.
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    Catalog:HG10250-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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