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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human BMP-5 ORF mammalian expression plasmid, C-GFPSpark tag||HG10214-ACG|
|Human BMP-5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10214-ACR|
|Human BMP-5 ORF mammalian expression plasmid, C-Flag tag||HG10214-CF|
|Human BMP-5 ORF mammalian expression plasmid, C-His tag||HG10214-CH|
|Human BMP-5 ORF mammalian expression plasmid, C-Myc tag||HG10214-CM|
|Human BMP-5 ORF mammalian expression plasmid, C-HA tag||HG10214-CY|
|Human BMP-5 ORF mammalian expression plasmid, Flag tag||HG10214-M-F|
|Human BMP-5 ORF mammalian expression plasmid, N-Flag tag||HG10214-NF|
|Human BMP-5 ORF mammalian expression plasmid, N-His tag||HG10214-NH|
|Human BMP-5 ORF mammalian expression plasmid, N-Myc tag||HG10214-NM|
|Human BMP-5 ORF mammalian expression plasmid, N-HA tag||HG10214-NY|
|Human BMP-5 natural ORF mammalian expression plasmid||HG10214-UT|
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Bone Morphogenetic Protein 5 (BMP-5) is a member of the structurally and functionally related bone morphogenetic proteins (BMPs) which constitute a novel subfamily of the transforming growth factor β (TGF-β) superfamily. In agreement with a possible role in the control of cell death, BMP-5 exhibited a regulated pattern of expression in the interdigital tissue. Transcripts of BMP-5 and BMP-5 protein were abundant within the cytoplasm of the fragmenting apoptotic interdigital cells in a way suggesting that delivery of BMPs into the tissue is potentiated during apoptosis. Gain-of-function experiments demonstrated that BMP-5 has the same effect as other interdigital BMPs inducing apoptosis in the undifferentiated mesoderm and growth in the prechondrogenic mesenchyme. BMP-5 is a member of the 60A subgroup of BMPs, other members of which have been shown to stimulate dendritic growth in central and peripheral neurons. The signaling pathway that mediates the dendrite-promoting activity of BMP-5 may involve binding to BMPR-IA and activation of Smad-1, and relative levels of BMP antagonists such as noggin and follistatin may modulate BMP-5 signaling. Since BMP-5 is expressed at relatively high levels not only in the developing but also the adult nervous system, these findings suggest the possibility that BMP-5 regulates dendritic morphology not only in the developing, but also the adult nervous system. BMP-5 may play important roles not only in myocardial differentiation, but also in the formation and maintenance of endocardial cushion tissue. Additionally, high expression level of BMP-5 has been detected in certain tumors of mesenchymal origin.