|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CCL22 / MDC ORF mammalian expression plasmid, C-GFPSpark tag||HG10163-ACG|
|Human CCL22 / MDC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10163-ACR|
|Human CCL22 / MDC ORF mammalian expression plasmid, C-Flag tag||HG10163-CF|
|Human CCL22 / MDC ORF mammalian expression plasmid, C-His tag||HG10163-CH|
|Human CCL22 / MDC ORF mammalian expression plasmid, C-Myc tag||HG10163-CM|
|Human CCL22 / MDC ORF mammalian expression plasmid, C-HA tag||HG10163-CY|
|Human CCL22 / MDC Gene cDNA clone plasmid||HG10163-G|
|Human CCL22 / MDC ORF mammalian expression plasmid, N-Flag tag||HG10163-NF|
|Human CCL22 / MDC ORF mammalian expression plasmid, N-His tag||HG10163-NH|
|Human CCL22 / MDC ORF mammalian expression plasmid, N-Myc tag||HG10163-NM|
|Human CCL22 / MDC ORF mammalian expression plasmid, N-HA tag||HG10163-NY|
|Human CCL22 / MDC natural ORF mammalian expression plasmid||HG10163-UT|
|Learn more about expression Vectors|
Chemokine (C-C motif) ligand 22(ABCD-1 / CCL22)is a kind of CC chemokine which is a family of secreted proteins involved in immunoregulatory and inflammatory processes. The cytokine displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. This ABCD-1 / CCL22 chemokine binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated / effector T lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. ABCD-1 / CCL22 is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus, and in Langerhans' cell histiocytosis and atopic dermatitis but not in dermatopathic lymphadenopathy. This chemokine is also found in lymph node, appendix, activated monocytes, resting and activated macrophages. This protein is lower expressed in lung and spleen and very weekly expressed in small intestine.