|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-GFPSpark tag||HG10128-ACG|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10128-ACR|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-Flag tag||HG10128-CF|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-His tag||HG10128-CH|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-Myc tag||HG10128-CM|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, C-HA tag||HG10128-CY|
|Human IL1F1 / IL1α Gene cDNA clone plasmid||HG10128-M|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, N-Flag tag||HG10128-NF|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, N-His tag||HG10128-NH|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, N-Myc tag||HG10128-NM|
|Human IL1F1 / IL1α ORF mammalian expression plasmid, N-HA tag||HG10128-NY|
|Human IL1F1 / IL1α natural ORF mammalian expression plasmid||HG10128-UT|
|Learn more about expression Vectors|
IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.