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Human CA VIII Gene cDNA Clone (full-length ORF Clone)

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CA8cDNA Clone Product Information
Gene_bank_ref_id:NM_004056.4
cDNA Size:873
cDNA Description:ORF Clone of Homo sapiens carbonic anhydrase VIII DNA.
Gene Synonym:CA8, CALS, CARP, CA-VIII
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 327 A/G not causing the amino acid variation.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human CA VIII Gene cDNA Clone (full-length ORF Clone) on other vectors
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10063-ACG$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10063-ACR$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10063-ANG$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10063-ANR$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10063-CF$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10063-CH$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10063-CM$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10063-CY$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG10063-M-F$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10063-M-N$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10063-NF$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10063-NH$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10063-NM$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10063-NY$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10063-UT$295
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Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
  • Size / Price
    Catalog:HG10063-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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