|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human EGFR ORF mammalian expression plasmid, C-GFPSpark tag||HG10001-ACG|
|Human EGFR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10001-ACR|
|Human EGFR ORF mammalian expression plasmid, C-His tag||HG10001-CH|
|Human EGFR ORF mammalian expression plasmid, C-Myc tag||HG10001-CM|
|Human EGFR ORF mammalian expression plasmid, N-Flag tag||HG10001-NF|
|Human EGFR ORF mammalian expression plasmid, N-His tag||HG10001-NH|
|Human EGFR ORF mammalian expression plasmid, N-Myc tag||HG10001-NM|
|Human EGFR ORF mammalian expression plasmid, N-HA tag||HG10001-NY|
|Human EGFR natural ORF mammalian expression plasmid||HG10001-UT|
|Learn more about expression Vectors|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.