|Datasheet||Specific References||Reviews||Related Products||Protocols|
|IL23A & IL12B|
|A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 85 % as determined by SDS-PAGE|
|Measured by its binding ability in a functional ELISA.|
Immobilized marmoset IL23A-His+marmoset IL12B-His (Cat:CT037-C08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H). The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 55-129 μg/ml.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|Arg 20 & Ile 23|
|The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE.|
|Lyophilized from sterile PBS, pH 7.4.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|