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Mouse IL-23 (IL23A & IL12B Heterodimer) Protein

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IL23A & IL12BProtein Product Information
Synonym:Interleukin-23 (IL23A & IL12B Heterodimer)
Protein Construction:A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
IL23A & IL12BProtein QC Testing
Purity:(33.1+31.5+32.9) % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA.
1. Measured by its ability to bind recombinant rat IL23R-Fc (Cat:80395-R02H) in a functional ELISA.
2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind human IL23R-Fc (Cat:13840-H02H). The EC50 of human IL23R-Fc (Cat:13840-H02H) is 0.14-0.35 μg/ml.
3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc (Cat:90123-C02H). The EC50 of Cynomolgus IL23R-Fc (Cat:90123-C02H) is 0.05-0.11 μg/ml.
4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H). The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 21.3-49.8 ng/mL.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Val 22 & Met 23
Molecule Mass:The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
IL23A & IL12BProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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  • Mouse IL-23 (IL23A & IL12B Heterodimer) Protein SDS-PAGE
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