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Human CDK7 & CCNH & MNAT1 Heterotrimer Protein

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Human CDK7 & CCNH & MNAT1 Protein Product Information
Synonym:CDK7 (cyclin-dependent kinase 7), CCNH (cyclin H) & MNAT1 (menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) ) Heterotrimer
Protein Construction:A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human CDK7 & CCNH & MNAT1 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 18 nmol/min/mg using MBP as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His & His & His
Molecule Mass:The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25,38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CDK7 & CCNH & MNAT1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CDK7 & CCNH & MNAT1 Protein SDS-PAGE
Human CDK7 & CCNH & MNAT1 Heterotrimer Protein SDS-PAGE
Human CDK7 & CCNH & MNAT1 Heterotrimer Protein SDS-PAGE
Other CDK7 & CCNH & MNAT1 Recombinant Protein Products
CT020-H07B
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Catalog: CT020-H07B-20
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"