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Human ITGAX & ITGB2 Heterodimer Protein

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Human ITGAX & ITGB2 Protein Product Information
Synonym:CD11C, SLEB6, LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Pro 1107) of human ITGAX (P20702) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asn 700) of human ITGB2 (P05107) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human ITGAX & ITGB2 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Phe 20 & Gln 21
Molecule Mass:The recombinant heterodimer of human ITGAX/ITGB2 comprises 1879 (1130+718) amino acids and has a calculated molecular mass of 205 (125+80) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGAX/ITGB2 heterodimer is approximately 95 &130 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ITGAX & ITGB2 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human ITGAX & ITGB2 Protein SDS-PAGE
Human ITGAX & ITGB2 Heterodimer Protein SDS-PAGE
Human ITGAX & ITGB2 Heterodimer Protein SDS-PAGE
Other ITGAX & ITGB2 Recombinant Protein Products
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Catalog: CT017-H2508H-50
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.