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Human ITGA6 & ITGB1 Heterodimer Protein

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Human ITGA6 & ITGB1 Protein Product Information
Synonym:CD49f, DKFZp686J01244, FLJ18737, ITGA6B, VLA-6, RP11-479G22.2, CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Ser 1011) of human ITGA6 isoform alpha-6X1B (P23229-3) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human ITGA6 & ITGB1 Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Phe 24 & Gln 21
Molecule Mass:The recombinant heterodimer of human ITGA6/ITGB1 comprises 1779 (1030 + 749) amino acids and has a calculated molecular mass of 198 (115 + 83) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGA6/ITGB1 heterodimer is approximately 110 & 130 kDa in SDS-PAGE under reducing conditions
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ITGA6 & ITGB1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human ITGA6 & ITGB1 Protein SDS-PAGE
Human ITGA6 & ITGB1 Heterodimer Protein SDS-PAGE
Other ITGA6 & ITGB1 Recombinant Protein Products
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Catalog: CT013-H2508H-20
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.