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Human AMPK (G1/B1/A2) Heterotrimer Protein

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AMPKProtein Product Information
Synonym:AMPK, 5' AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 2 catalytic subunit, beta 1 non-catalytic subunit and gamma 1 non-catalytic subunit.
Protein Construction:A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:Liquid. It is shipped out with blue ice.
AMPKProtein QC Testing
Purity:> 94 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate (see protocol for kinase assay).
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met & Met & His
Molecule Mass:The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
AMPKProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
CT008-H0907B
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Catalog:CT008-H0907B-20
List Price: $138.00  (Save $0.00)
Price:$138.00      [How to order]
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  • Human AMPK (G1/B1/A2) Heterotrimer Protein SDS-PAGE
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