|Datasheet||Specific References||Reviews||Related Products||Protocols|
|AMPK, 5' AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 2 catalytic subunit, beta 1 non-catalytic subunit and gamma 1 non-catalytic subunit.|
|A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.|
|Liquid. It is shipped out with blue ice.|
|> 94 % as determined by SDS-PAGE|
|The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate (see protocol for kinase assay).|
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|Met & Met & His|
|The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions.|
|Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|