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Cynomolgus monkey PTGFRN Gene cDNA clone plasmid

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Cynomolgus PTGFRN cDNA Clone Product Information
Gene_bank_ref_id:unsubmitted
RefSeq ORF Size:2640bp
cDNA Description:Full length Clone DNA of Macaca mulatta prostaglandin F2 receptor negative regulator.
Gene Synonym:PTGFRN
Species:Cynomolgus
Vector:pGEM-T Vector
Plasmid:pGEM-cynoPTGFRN
Restriction Site:
Tag Sequence:
Sequence Description:Identical with XM_001103382.2 [ Macaca mulatta (Rhesus monkey) ]: 1151 A/G resulting in the amino acid Lys substitution by Arg and 162 G/A, 525 G/A, 1719 A/G, 1776 T/C, 2052 G/A not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Product nameProduct name
Background

EWI-F, also known as PTGFRN, is inversely related to the loss of CD9. Its expression correlates with the metastatic status of hLT (n=55). EWI-F inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. EWI-F expression positively correlates with the metastatic status of hLT, and that the upregulation of EWI-F expression could be one of the mechanisms underlying the loss of CD9 in solid tumours.

References
  • Stipp C S. et al., 2001, J Biol Chem. 276 (7): 4853-62.
  • Charrin S. et al., 2001, J Biol Chem. 276 (17): 14329-37.
  • Orlicky DJ. et al., 1996, Hum Genet. 97 (5): 655-8.
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    Catalog: CG90276-G
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"