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Goat Anti-Human IgG Secondary Antibody (HRP)

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Overview
 

Description:

Goat polyclonal Secondary Antibody to Human IgG (HRP).

Host

Goat

Target species:

Human IgG

Specificity:

By ELISA this antibody reacts specifically with Human IgG.

 

Has cross-reactivity with rabbit IgG, rat IgG (>10%), horse IgG (3.5%), guinea pig IgG (3.6%), human IgM (2.2%)

Minimal cross-reactivity with mouse IgG, bovine IgG

Applications:

ICC, IHC-P, ELISA, WB

 
Properties

Form:

Liquid

Storage & Stability:

Do not freeze! Store at 4℃ and protected from prolonged exposure to light. Antibody products are stable for six months from date of receipt when stored at 2℃ to 8℃.

Storage buffer: Preservative: 0.1% Proclin

Constituents:

0.2% BSA, PBS

Purification notes:

Immunogen affinity purified

Formulation:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

ICC

Use at an assay dependent dilution

IHC-P

1/200 - 1/2000

ELISA

1/10000 - 1/100000 (Primary)

 WB

1/2000 - 1/25000

(Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

 
Size / Price
Catalog: SSA002-200
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"