Normal Guinea Pig Control IgG is essential for ELISA, Western Blot (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP) experiments. It's purpose is to estimate that the proteins stained in the experiment result are due to the specific interaction with the antibody. Some people use specific primary antibody alone. This does not consider of those proteins that may bind to regions of the immunoglobulin distinct from the specific antigen binding sites. This Non-Specific binding is due to Fc receptor binding or other protein-protein interactions.The best way is to use isotype-matched control for the experiment. The IgG isotyping control should have the same immunoglobulin subtype and be used at the same concentration as the specific detection antibody. Sino Biological has a very good selection of control IgGs and the quality is excellent.
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