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Normal Goat Control IgG

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    Catalog:CR2-500
    List Price: $35.00  (Save $0.00)
    Price:$35.00      [How to order]
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    Normal Goat Control IgG Antibody Product Information
     
    Antibody Type : Goat Polyclonal Antibody
    Ig Type : Goat IgG
    Reactivity : Goat
    Formulation : 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4
    Source/purification: The antibody was isolated from normal Goat serum and purified by protein A.
    Specificity : The purified antibody is not directed against any known antigen. (No known specificity)
     
    Normal Goat Control IgG Usage Guide
     
    Application : ELISA, WB, IHC, IP
    This control antibody must be diluted to the same concentration as the specific primary antibody used for analysis.
    Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
     
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    Additional Primary & Secondary Antibody Reagents
     
    Normal Goat Control IgG Background

    Normal Goat Control IgG is essential for ELISA, Western Blot (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP) experiments. It's purpose is to estimate that the proteins stained in the experiment result are due to the specific interaction with the antibody. Some people use specific primary antibody alone. This does not consider of those proteins that may bind to regions of the immunoglobulin distinct from the specific antigen binding sites. This Non-Specific binding is due to Fc receptor binding or other protein-protein interactions.The best way is to use isotype-matched control for the experiment. The IgG isotyping control should have the same immunoglobulin subtype and be used at the same concentration as the specific detection antibody. Sino Biological has a very good selection of control IgGs and the quality is excellent.

     
    References
    1. Amigorena S. et al., 1998, J Exp Med. 187 (4): 505-15.
    2. Hurez V. et al., 1993, Eur J Immunol. 23 (4): 783-9.
    3. Johnson RJ. et al., 1992, J Exp Med. 175 (5): 1413-6.
     
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"