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Normal Rabbit Control IgG

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Normal Rabbit Control IgG Product Information
 
Antibody Type : Rabbit Polyclonal Antibody
Ig Type : Rabbit IgG
Reactivity : Rabbit
Formulation : 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4
Source/
purification:
The antibody was isolated from normal rabbit serum and purified by protein A.
Specificity : The purified antibody is not directed against any known antigen. (No known specificity)
 
Normal Rabbit Control IgG Usage Guide
 
Application : ELISA, WB, IHC, IP
This control antibody must be diluted to the same concentration as the specific primary antibody used for analysis.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
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Additional Primary & Secondary Antibody Reagents
 
Normal Rabbit Control IgG Background

Normal Rabbit Control IgG is essential for ELISA, Western Blot (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP) experiments. It's purpose is to estimate that the proteins stained in the experiment result are due to the specific interaction with the antibody. Some people use specific primary antibody alone. This does not consider of those proteins that may bind to regions of the immunoglobulin distinct from the specific antigen binding sites. This Non-Specific binding is due to Fc receptor binding or other protein-protein interactions.The best way is to use isotype-matched control for the experiment. The IgG isotyping control should have the same immunoglobulin subtype and be used at the same concentration as the specific detection antibody. Sino Biological has a very good selection of control IgGs and the quality is excellent.

References
 
  1. Amigorena S. et al., 1998, J Exp Med. 187 (4): 505-15.
  2. Hurez V. et al., 1993, Eur J Immunol. 23 (4): 783-9.
  3. Johnson RJ. et al., 1992, J Exp Med. 175 (5): 1413-6.
 

 

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Catalog: CR1-100
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