|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse WIF1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50984-ACG|
|Mouse WIF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50984-ACR|
|Mouse WIF1 ORF mammalian expression plasmid, C-Flag tag||MG50984-CF|
|Mouse WIF1 ORF mammalian expression plasmid, C-His tag||MG50984-CH|
|Mouse WIF1 ORF mammalian expression plasmid, C-Myc tag||MG50984-CM|
|Mouse WIF1 ORF mammalian expression plasmid, C-HA tag||MG50984-CY|
|Mouse WIF1 Gene cDNA clone plasmid||MG50984-G|
|Mouse WIF1 ORF mammalian expression plasmid, N-Flag tag||MG50984-NF|
|Mouse WIF1 ORF mammalian expression plasmid, N-His tag||MG50984-NH|
|Mouse WIF1 ORF mammalian expression plasmid, N-Myc tag||MG50984-NM|
|Mouse WIF1 ORF mammalian expression plasmid, N-HA tag||MG50984-NY|
|Mouse WIF1 natural ORF mammalian expression plasmid||MG50984-UT|
|Learn more about expression Vectors|
WIF1, also known as WIF-1 and wnt inhibitory factor 1, is a secreted protein which binds WNT proteins and inhibits their activities. It contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 may be involved in mesoderm segmentation and can be detected in fish, amphibia and mammals. WIF-1 is a recurrent target in human salivary gland oncogenesis. Downregulation of WIF1 takes part in the development and progression of pleomorphic adenomas. WIF1 also is a tumor suppressor, and has been found to be epigenetically silenced in various cancers, specifically in nonfunctioning pituitary tumors. WIF1 are expected to have molecular function (protein tyrosine kinase activity) and to localize in various compartments (extracellular space, extracellular region).