|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse WTAP ORF mammalian expression plasmid, C-GFPSpark tag||MG52198-ACG|
|Mouse WTAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52198-ACR|
|Mouse WTAP ORF mammalian expression plasmid, N-GFPSpark tag||MG52198-ANG|
|Mouse WTAP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52198-ANR|
|Mouse WTAP ORF mammalian expression plasmid, C-Flag tag||MG52198-CF|
|Mouse WTAP ORF mammalian expression plasmid, C-His tag||MG52198-CH|
|Mouse WTAP ORF mammalian expression plasmid, C-Myc tag||MG52198-CM|
|Mouse WTAP ORF mammalian expression plasmid, C-HA tag||MG52198-CY|
|Mouse WTAP Gene cDNA clone plasmid||MG52198-G|
|Mouse WTAP ORF mammalian expression plasmid, N-Flag tag||MG52198-NF|
|Mouse WTAP ORF mammalian expression plasmid, N-His tag||MG52198-NH|
|Mouse WTAP ORF mammalian expression plasmid, N-Myc tag||MG52198-NM|
|Mouse WTAP ORF mammalian expression plasmid, N-HA tag||MG52198-NY|
|Mouse WTAP natural ORF mammalian expression plasmid||MG52198-UT|
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Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.