|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52196-ACG|
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52196-ACR|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-GFPSpark tag||MG52196-ANG|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52196-ANR|
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-Flag tag||MG52196-CF|
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-His tag||MG52196-CH|
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-Myc tag||MG52196-CM|
|Mouse ALDH7A1 ORF mammalian expression plasmid, C-HA tag||MG52196-CY|
|Mouse ALDH7A1 Gene cDNA clone plasmid||MG52196-G|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-Flag tag||MG52196-NF|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-His tag||MG52196-NH|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-Myc tag||MG52196-NM|
|Mouse ALDH7A1 ORF mammalian expression plasmid, N-HA tag||MG52196-NY|
|Mouse ALDH7A1 natural ORF mammalian expression plasmid||MG52196-UT|
|Learn more about expression Vectors|
ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.