|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse KLK-8 ORF mammalian expression plasmid, C-GFPSpark tag||MG50967-ACG|
|Mouse KLK-8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50967-ACR|
|Mouse KLK-8 ORF mammalian expression plasmid, C-Flag tag||MG50967-CF|
|Mouse KLK-8 ORF mammalian expression plasmid, C-His tag||MG50967-CH|
|Mouse KLK-8 ORF mammalian expression plasmid, C-Myc tag||MG50967-CM|
|Mouse KLK-8 ORF mammalian expression plasmid, C-HA tag||MG50967-CY|
|Mouse KLK-8 Gene cDNA clone plasmid||MG50967-G|
|Mouse KLK-8 ORF mammalian expression plasmid, N-Flag tag||MG50967-NF|
|Mouse KLK-8 ORF mammalian expression plasmid, N-His tag||MG50967-NH|
|Mouse KLK-8 ORF mammalian expression plasmid, N-Myc tag||MG50967-NM|
|Mouse KLK-8 ORF mammalian expression plasmid, N-HA tag||MG50967-NY|
|Mouse KLK-8 natural ORF mammalian expression plasmid||MG50967-UT|
|Learn more about expression Vectors|
Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein and KLK8, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. It is a serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Kallikrein-8 / KLK8 plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. It regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. It is involved in skin desquamation and keratinocyte proliferation and plays a role in the secondary phase of pathogenesis following spinal cord injury. It also cleaves L1CAM in response to increased neural activity. It induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Kallikrein-8 / KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. Kallikrein-8 / KLK8 may have potential clinical value for disease diagnosis or prognosis and it may also be a useful therapeutic target.