|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PLS3 ORF mammalian expression plasmid, C-GFPSpark tag||MG52182-ACG|
|Mouse PLS3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52182-ACR|
|Mouse PLS3 ORF mammalian expression plasmid, N-GFPSpark tag||MG52182-ANG|
|Mouse PLS3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52182-ANR|
|Mouse PLS3 ORF mammalian expression plasmid, C-Flag tag||MG52182-CF|
|Mouse PLS3 ORF mammalian expression plasmid, C-His tag||MG52182-CH|
|Mouse PLS3 ORF mammalian expression plasmid, C-Myc tag||MG52182-CM|
|Mouse PLS3 ORF mammalian expression plasmid, C-HA tag||MG52182-CY|
|Mouse PLS3 Gene cDNA clone plasmid||MG52182-G|
|Mouse PLS3 ORF mammalian expression plasmid, N-Flag tag||MG52182-NF|
|Mouse PLS3 ORF mammalian expression plasmid, N-His tag||MG52182-NH|
|Mouse PLS3 ORF mammalian expression plasmid, N-Myc tag||MG52182-NM|
|Mouse PLS3 ORF mammalian expression plasmid, N-HA tag||MG52182-NY|
|Mouse PLS3 natural ORF mammalian expression plasmid||MG52182-UT|
|Learn more about expression Vectors|
PLS3, also known as plastin 3, belongs to the plastin family. Members of this family are actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. There are two ubiquitous plastin isoforms in humans: L and T. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). PLS3 contains 2 actin-binding domains, 4 CH (calponin-homology) domains and 2 EF-hand domains. It is expressed in a variety of organs, including muscle, brain, uterus and esophagus.