|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse B2M ORF mammalian expression plasmid, C-GFPSpark tag||MG50957-ACG|
|Mouse B2M ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50957-ACR|
|Mouse B2M ORF mammalian expression plasmid, C-Flag tag||MG50957-CF|
|Mouse B2M ORF mammalian expression plasmid, C-His tag||MG50957-CH|
|Mouse B2M ORF mammalian expression plasmid, C-Myc tag||MG50957-CM|
|Mouse B2M ORF mammalian expression plasmid, C-HA tag||MG50957-CY|
|Mouse B2M Gene cDNA clone plasmid||MG50957-G|
|Mouse B2M ORF mammalian expression plasmid, N-Flag tag||MG50957-NF|
|Mouse B2M ORF mammalian expression plasmid, N-His tag||MG50957-NH|
|Mouse B2M ORF mammalian expression plasmid, N-Myc tag||MG50957-NM|
|Mouse B2M ORF mammalian expression plasmid, N-HA tag||MG50957-NY|
|Mouse B2M natural ORF mammalian expression plasmid||MG50957-UT|
|Learn more about expression Vectors|
B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.