|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CBFB ORF mammalian expression plasmid, C-GFPSpark tag||MG52175-ACG|
|Mouse CBFB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52175-ACR|
|Mouse CBFB ORF mammalian expression plasmid, N-GFPSpark tag||MG52175-ANG|
|Mouse CBFB ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52175-ANR|
|Mouse CBFB ORF mammalian expression plasmid, C-Flag tag||MG52175-CF|
|Mouse CBFB ORF mammalian expression plasmid, C-His tag||MG52175-CH|
|Mouse CBFB ORF mammalian expression plasmid, C-Myc tag||MG52175-CM|
|Mouse CBFB ORF mammalian expression plasmid, C-HA tag||MG52175-CY|
|Mouse CBFB Gene cDNA clone plasmid||MG52175-G|
|Mouse CBFB ORF mammalian expression plasmid, N-Flag tag||MG52175-NF|
|Mouse CBFB ORF mammalian expression plasmid, N-His tag||MG52175-NH|
|Mouse CBFB ORF mammalian expression plasmid, N-Myc tag||MG52175-NM|
|Mouse CBFB ORF mammalian expression plasmid, N-HA tag||MG52175-NY|
|Mouse CBFB natural ORF mammalian expression plasmid||MG52175-UT|
|Learn more about expression Vectors|
CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). CBFB is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for CBFB gene. Mutations in CBFB are implicated in cases of breast cancer.