|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TIGIT ORF mammalian expression plasmid, C-GFPSpark tag||MG50939-ACG|
|Mouse TIGIT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50939-ACR|
|Mouse TIGIT ORF mammalian expression plasmid, C-Flag tag||MG50939-CF|
|Mouse TIGIT ORF mammalian expression plasmid, C-His tag||MG50939-CH|
|Mouse TIGIT ORF mammalian expression plasmid, C-Myc tag||MG50939-CM|
|Mouse TIGIT ORF mammalian expression plasmid, C-HA tag||MG50939-CY|
|Mouse TIGIT Gene cDNA clone plasmid||MG50939-G|
|Mouse TIGIT ORF mammalian expression plasmid, N-Flag tag||MG50939-NF|
|Mouse TIGIT ORF mammalian expression plasmid, N-His tag||MG50939-NH|
|Mouse TIGIT ORF mammalian expression plasmid, N-Myc tag||MG50939-NM|
|Mouse TIGIT ORF mammalian expression plasmid, N-HA tag||MG50939-NY|
|Mouse TIGIT natural ORF mammalian expression plasmid||MG50939-UT|
|Learn more about expression Vectors|
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.