|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CHI3L1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50929-ACG|
|Mouse CHI3L1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50929-ACR|
|Mouse CHI3L1 ORF mammalian expression plasmid, C-Flag tag||MG50929-CF|
|Mouse CHI3L1 ORF mammalian expression plasmid, C-His tag||MG50929-CH|
|Mouse CHI3L1 ORF mammalian expression plasmid, C-Myc tag||MG50929-CM|
|Mouse CHI3L1 ORF mammalian expression plasmid, C-HA tag||MG50929-CY|
|Mouse CHI3L1 Gene cDNA clone plasmid||MG50929-G|
|Mouse CHI3L1 ORF mammalian expression plasmid, N-Flag tag||MG50929-NF|
|Mouse CHI3L1 ORF mammalian expression plasmid, N-His tag||MG50929-NH|
|Mouse CHI3L1 ORF mammalian expression plasmid, N-Myc tag||MG50929-NM|
|Mouse CHI3L1 ORF mammalian expression plasmid, N-HA tag||MG50929-NY|
|Mouse CHI3L1 natural ORF mammalian expression plasmid||MG50929-UT|
|Learn more about expression Vectors|
Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.