|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TACSTD2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50922-ACG|
|Mouse TACSTD2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50922-ACR|
|Mouse TACSTD2 ORF mammalian expression plasmid, C-Flag tag||MG50922-CF|
|Mouse TACSTD2 ORF mammalian expression plasmid, C-His tag||MG50922-CH|
|Mouse TACSTD2 ORF mammalian expression plasmid, C-Myc tag||MG50922-CM|
|Mouse TACSTD2 ORF mammalian expression plasmid, C-HA tag||MG50922-CY|
|Mouse TACSTD2 Gene cDNA clone plasmid||MG50922-G|
|Mouse TACSTD2 ORF mammalian expression plasmid, N-Flag tag||MG50922-NF|
|Mouse TACSTD2 ORF mammalian expression plasmid, N-His tag||MG50922-NH|
|Mouse TACSTD2 ORF mammalian expression plasmid, N-Myc tag||MG50922-NM|
|Mouse TACSTD2 ORF mammalian expression plasmid, N-HA tag||MG50922-NY|
|Mouse TACSTD2 natural ORF mammalian expression plasmid||MG50922-UT|
|Learn more about expression Vectors|
TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.