|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CCDC47 ORF mammalian expression plasmid, C-GFPSpark tag||MG52105-ACG|
|Mouse CCDC47 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52105-ACR|
|Mouse CCDC47 ORF mammalian expression plasmid, N-GFPSpark tag||MG52105-ANG|
|Mouse CCDC47 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52105-ANR|
|Mouse CCDC47 ORF mammalian expression plasmid, C-Flag tag||MG52105-CF|
|Mouse CCDC47 ORF mammalian expression plasmid, C-His tag||MG52105-CH|
|Mouse CCDC47 ORF mammalian expression plasmid, C-Myc tag||MG52105-CM|
|Mouse CCDC47 ORF mammalian expression plasmid, C-HA tag||MG52105-CY|
|Mouse CCDC47 ORF mammalian expression plasmid, N-Flag tag||MG52105-NF|
|Mouse CCDC47 ORF mammalian expression plasmid, N-His tag||MG52105-NH|
|Mouse CCDC47 ORF mammalian expression plasmid, N-Myc tag||MG52105-NM|
|Mouse CCDC47 ORF mammalian expression plasmid, N-HA tag||MG52105-NY|
|Mouse CCDC47 Gene cDNA clone plasmid||MG52105-U|
|Mouse CCDC47 natural ORF mammalian expression plasmid||MG52105-UT|
|Learn more about expression Vectors|
CCDC47 gene is expressed at high level. The gene contains 16 distinct gt-ag introns. Transcription produces 9 different mRNAs, 6 alternatively spliced variants and 3 unspliced forms. There are 3 probable alternative promotors, 3 non overlapping alternative last exons and 8 validated alternative polyadenylation sites. The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon, overlapping exons with different boundaries. Functionally, CCDC47 gene has been proposed to participate in processes such as calcium ion homeostasis, embryo development, ER overload response and post-embryonic development. CCDC47 are expected to have molecular function (calcium ion binding) and to localize in various compartments (membrane, endoplasmic reticulum, integral to membrane, microsome).