|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PHGDH ORF mammalian expression plasmid, C-GFPSpark tag||MG51754-ACG|
|Mouse PHGDH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51754-ACR|
|Mouse PHGDH ORF mammalian expression plasmid, N-GFPSpark tag||MG51754-ANG|
|Mouse PHGDH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51754-ANR|
|Mouse PHGDH ORF mammalian expression plasmid, C-Flag tag||MG51754-CF|
|Mouse PHGDH ORF mammalian expression plasmid, C-His tag||MG51754-CH|
|Mouse PHGDH ORF mammalian expression plasmid, C-Myc tag||MG51754-CM|
|Mouse PHGDH ORF mammalian expression plasmid, C-HA tag||MG51754-CY|
|Mouse PHGDH ORF mammalian expression plasmid, N-Flag tag||MG51754-NF|
|Mouse PHGDH ORF mammalian expression plasmid, N-His tag||MG51754-NH|
|Mouse PHGDH ORF mammalian expression plasmid, N-Myc tag||MG51754-NM|
|Mouse PHGDH ORF mammalian expression plasmid, N-HA tag||MG51754-NY|
|Mouse PHGDH Gene cDNA clone plasmid||MG51754-U|
|Mouse PHGDH natural ORF mammalian expression plasmid||MG51754-UT|
|Learn more about expression Vectors|
PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.