|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse NEK3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50880-ACG|
|Mouse NEK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50880-ACR|
|Mouse NEK3 ORF mammalian expression plasmid, N-GFPSpark tag||MG50880-ANG|
|Mouse NEK3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50880-ANR|
|Mouse NEK3 ORF mammalian expression plasmid, C-Flag tag||MG50880-CF|
|Mouse NEK3 ORF mammalian expression plasmid, C-His tag||MG50880-CH|
|Mouse NEK3 ORF mammalian expression plasmid, C-Myc tag||MG50880-CM|
|Mouse NEK3 ORF mammalian expression plasmid, C-HA tag||MG50880-CY|
|Mouse NEK3 Gene cDNA clone plasmid||MG50880-G|
|Mouse NEK3 ORF mammalian expression plasmid, N-Flag tag||MG50880-NF|
|Mouse NEK3 ORF mammalian expression plasmid, N-His tag||MG50880-NH|
|Mouse NEK3 ORF mammalian expression plasmid, N-Myc tag||MG50880-NM|
|Mouse NEK3 ORF mammalian expression plasmid, N-HA tag||MG50880-NY|
|Mouse NEK3 natural ORF mammalian expression plasmid||MG50880-UT|
|Learn more about expression Vectors|
NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.