|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TPP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52010-ACG|
|Mouse TPP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52010-ACR|
|Mouse TPP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG52010-ANG|
|Mouse TPP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52010-ANR|
|Mouse TPP1 ORF mammalian expression plasmid, C-Flag tag||MG52010-CF|
|Mouse TPP1 ORF mammalian expression plasmid, C-His tag||MG52010-CH|
|Mouse TPP1 ORF mammalian expression plasmid, C-Myc tag||MG52010-CM|
|Mouse TPP1 ORF mammalian expression plasmid, C-HA tag||MG52010-CY|
|Mouse TPP1 Gene cDNA clone plasmid||MG52010-G|
|Mouse TPP1 ORF mammalian expression plasmid, N-Flag tag||MG52010-NF|
|Mouse TPP1 ORF mammalian expression plasmid, N-His tag||MG52010-NH|
|Mouse TPP1 ORF mammalian expression plasmid, N-Myc tag||MG52010-NM|
|Mouse TPP1 ORF mammalian expression plasmid, N-HA tag||MG52010-NY|
|Mouse TPP1 Gene cDNA clone plasmid||MG52010-U|
|Mouse TPP1 natural ORF mammalian expression plasmid||MG52010-UT|
|Learn more about expression Vectors|
Tripeptidyl-peptidase 1 (TPP1 / CLN2) is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. TPP1 / CLN2 May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Defects in TPP1 / CLN2 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2), a form of neuronal ceroid lipofuscinosis which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.