|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse GRK5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50860-ACG|
|Mouse GRK5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50860-ACR|
|Mouse GRK5 ORF mammalian expression plasmid, N-GFPSpark tag||MG50860-ANG|
|Mouse GRK5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50860-ANR|
|Mouse GRK5 ORF mammalian expression plasmid, C-Flag tag||MG50860-CF|
|Mouse GRK5 ORF mammalian expression plasmid, C-His tag||MG50860-CH|
|Mouse GRK5 ORF mammalian expression plasmid, C-Myc tag||MG50860-CM|
|Mouse GRK5 ORF mammalian expression plasmid, C-HA tag||MG50860-CY|
|Mouse GRK5 Gene cDNA clone plasmid||MG50860-G|
|Mouse GRK5 ORF mammalian expression plasmid, N-Flag tag||MG50860-NF|
|Mouse GRK5 ORF mammalian expression plasmid, N-His tag||MG50860-NH|
|Mouse GRK5 ORF mammalian expression plasmid, N-Myc tag||MG50860-NM|
|Mouse GRK5 ORF mammalian expression plasmid, N-HA tag||MG50860-NY|
|Mouse GRK5 natural ORF mammalian expression plasmid||MG50860-UT|
|Learn more about expression Vectors|
G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors.
GRK5 contains one AGC-kinase C-terminal domain, one protein kinase domain and one RGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.