|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PDE4B ORF mammalian expression plasmid, C-GFPSpark tag||MG51965-ACG|
|Mouse PDE4B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51965-ACR|
|Mouse PDE4B ORF mammalian expression plasmid, N-GFPSpark tag||MG51965-ANG|
|Mouse PDE4B ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51965-ANR|
|Mouse PDE4B ORF mammalian expression plasmid, C-Flag tag||MG51965-CF|
|Mouse PDE4B ORF mammalian expression plasmid, C-His tag||MG51965-CH|
|Mouse PDE4B ORF mammalian expression plasmid, C-Myc tag||MG51965-CM|
|Mouse PDE4B ORF mammalian expression plasmid, C-HA tag||MG51965-CY|
|Mouse PDE4B Gene cDNA clone plasmid||MG51965-G|
|Mouse PDE4B ORF mammalian expression plasmid, N-Flag tag||MG51965-NF|
|Mouse PDE4B ORF mammalian expression plasmid, N-His tag||MG51965-NH|
|Mouse PDE4B ORF mammalian expression plasmid, N-Myc tag||MG51965-NM|
|Mouse PDE4B ORF mammalian expression plasmid, N-HA tag||MG51965-NY|
|Mouse PDE4B natural ORF mammalian expression plasmid||MG51965-UT|
|Learn more about expression Vectors|
cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents