|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse OTUB1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51912-ACG|
|Mouse OTUB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51912-ACR|
|Mouse OTUB1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51912-ANG|
|Mouse OTUB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51912-ANR|
|Mouse OTUB1 ORF mammalian expression plasmid, C-Flag tag||MG51912-CF|
|Mouse OTUB1 ORF mammalian expression plasmid, C-His tag||MG51912-CH|
|Mouse OTUB1 ORF mammalian expression plasmid, C-Myc tag||MG51912-CM|
|Mouse OTUB1 ORF mammalian expression plasmid, C-HA tag||MG51912-CY|
|Mouse OTUB1 Gene cDNA clone plasmid||MG51912-G|
|Mouse OTUB1 ORF mammalian expression plasmid, N-Flag tag||MG51912-NF|
|Mouse OTUB1 ORF mammalian expression plasmid, N-His tag||MG51912-NH|
|Mouse OTUB1 ORF mammalian expression plasmid, N-Myc tag||MG51912-NM|
|Mouse OTUB1 ORF mammalian expression plasmid, N-HA tag||MG51912-NY|
|Mouse OTUB1 natural ORF mammalian expression plasmid||MG51912-UT|
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Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to the peptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128 / GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.