|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CFL2 ORF mammalian expression plasmid, C-GFPSpark tag||MG51672-ACG|
|Mouse CFL2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51672-ACR|
|Mouse CFL2 ORF mammalian expression plasmid, N-GFPSpark tag||MG51672-ANG|
|Mouse CFL2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51672-ANR|
|Mouse CFL2 ORF mammalian expression plasmid, C-Flag tag||MG51672-CF|
|Mouse CFL2 ORF mammalian expression plasmid, C-His tag||MG51672-CH|
|Mouse CFL2 ORF mammalian expression plasmid, C-Myc tag||MG51672-CM|
|Mouse CFL2 ORF mammalian expression plasmid, C-HA tag||MG51672-CY|
|Mouse CFL2 Gene cDNA clone plasmid||MG51672-G|
|Mouse CFL2 ORF mammalian expression plasmid, N-Flag tag||MG51672-NF|
|Mouse CFL2 ORF mammalian expression plasmid, N-His tag||MG51672-NH|
|Mouse CFL2 ORF mammalian expression plasmid, N-Myc tag||MG51672-NM|
|Mouse CFL2 ORF mammalian expression plasmid, N-HA tag||MG51672-NY|
|Mouse CFL2 natural ORF mammalian expression plasmid||MG51672-UT|
|Learn more about expression Vectors|
Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.