|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PTPN1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51869-ACG|
|Mouse PTPN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51869-ACR|
|Mouse PTPN1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51869-ANG|
|Mouse PTPN1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51869-ANR|
|Mouse PTPN1 ORF mammalian expression plasmid, C-Flag tag||MG51869-CF|
|Mouse PTPN1 ORF mammalian expression plasmid, C-His tag||MG51869-CH|
|Mouse PTPN1 ORF mammalian expression plasmid, C-Myc tag||MG51869-CM|
|Mouse PTPN1 ORF mammalian expression plasmid, C-HA tag||MG51869-CY|
|Mouse PTPN1 Gene cDNA clone plasmid||MG51869-G|
|Mouse PTPN1 ORF mammalian expression plasmid, N-Flag tag||MG51869-NF|
|Mouse PTPN1 ORF mammalian expression plasmid, N-His tag||MG51869-NH|
|Mouse PTPN1 ORF mammalian expression plasmid, N-Myc tag||MG51869-NM|
|Mouse PTPN1 ORF mammalian expression plasmid, N-HA tag||MG51869-NY|
|Mouse PTPN1 natural ORF mammalian expression plasmid||MG51869-UT|
|Learn more about expression Vectors|
PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.