|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse EIF2C2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50683-ACG|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50683-ACR|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-GFPSpark tag||MG50683-ANG|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50683-ANR|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-Flag tag||MG50683-CF|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-His tag||MG50683-CH|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-Myc tag||MG50683-CM|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-HA tag||MG50683-CY|
|Mouse EIF2C2 Gene cDNA clone plasmid||MG50683-M|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-Flag tag||MG50683-NF|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-His tag||MG50683-NH|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-Myc tag||MG50683-NM|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-HA tag||MG50683-NY|
|Mouse EIF2C2 natural ORF mammalian expression plasmid||MG50683-UT|
|Learn more about expression Vectors|
Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.