|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CAR9 ORF mammalian expression plasmid, C-GFPSpark tag||MG50660-ACG|
|Mouse CAR9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50660-ACR|
|Mouse CAR9 ORF mammalian expression plasmid, C-Flag tag||MG50660-CF|
|Mouse CAR9 ORF mammalian expression plasmid, C-His tag||MG50660-CH|
|Mouse CAR9 ORF mammalian expression plasmid, C-Myc tag||MG50660-CM|
|Mouse CAR9 ORF mammalian expression plasmid, C-HA tag||MG50660-CY|
|Mouse CAR9 Gene cDNA clone plasmid||MG50660-G|
|Mouse CAR9 ORF mammalian expression plasmid, N-Flag tag||MG50660-NF|
|Mouse CAR9 ORF mammalian expression plasmid, N-His tag||MG50660-NH|
|Mouse CAR9 ORF mammalian expression plasmid, N-Myc tag||MG50660-NM|
|Mouse CAR9 ORF mammalian expression plasmid, N-HA tag||MG50660-NY|
|Mouse CAR9 natural ORF mammalian expression plasmid||MG50660-UT|
|Learn more about expression Vectors|
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.