|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TLR4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50657-ACG|
|Mouse TLR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50657-ACR|
|Mouse TLR4 ORF mammalian expression plasmid, C-Flag tag||MG50657-CF|
|Mouse TLR4 ORF mammalian expression plasmid, C-His tag||MG50657-CH|
|Mouse TLR4 ORF mammalian expression plasmid, C-Myc tag||MG50657-CM|
|Mouse TLR4 ORF mammalian expression plasmid, C-HA tag||MG50657-CY|
|Mouse TLR4 Gene cDNA clone plasmid||MG50657-M|
|Mouse TLR4 ORF mammalian expression plasmid, N-Flag tag||MG50657-NF|
|Mouse TLR4 ORF mammalian expression plasmid, N-His tag||MG50657-NH|
|Mouse TLR4 ORF mammalian expression plasmid, N-Myc tag||MG50657-NM|
|Mouse TLR4 ORF mammalian expression plasmid, N-HA tag||MG50657-NY|
|Mouse TLR4 natural ORF mammalian expression plasmid||MG50657-UT|
|Learn more about expression Vectors|
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).