|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CCL7 ORF mammalian expression plasmid, C-GFPSpark tag||MG50627-ACG|
|Mouse CCL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50627-ACR|
|Mouse CCL7 ORF mammalian expression plasmid, C-Flag tag||MG50627-CF|
|Mouse CCL7 ORF mammalian expression plasmid, C-His tag||MG50627-CH|
|Mouse CCL7 ORF mammalian expression plasmid, C-Myc tag||MG50627-CM|
|Mouse CCL7 ORF mammalian expression plasmid, C-HA tag||MG50627-CY|
|Mouse CCL7 Gene cDNA clone plasmid||MG50627-M|
|Mouse CCL7 ORF mammalian expression plasmid, N-Flag tag||MG50627-NF|
|Mouse CCL7 ORF mammalian expression plasmid, N-His tag||MG50627-NH|
|Mouse CCL7 ORF mammalian expression plasmid, N-Myc tag||MG50627-NM|
|Mouse CCL7 ORF mammalian expression plasmid, N-HA tag||MG50627-NY|
|Mouse CCL7 natural ORF mammalian expression plasmid||MG50627-UT|
|Learn more about expression Vectors|
Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 7(CCL7), also known as MCP-3, is a isform of the C-C chemokine subfamily of the chemokine family which is produced by certain tumor cells and by macrophages. It also own two adjacent N-terminal cysteine residues. Chemokine ligand 7(CCL7) spacifically attracts monocytes, and regulates macrophage function.