|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SCG3 ORF mammalian expression plasmid, C-GFPSpark tag||MG51561-ACG|
|Mouse SCG3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51561-ACR|
|Mouse SCG3 ORF mammalian expression plasmid, C-Flag tag||MG51561-CF|
|Mouse SCG3 ORF mammalian expression plasmid, C-His tag||MG51561-CH|
|Mouse SCG3 ORF mammalian expression plasmid, C-Myc tag||MG51561-CM|
|Mouse SCG3 ORF mammalian expression plasmid, C-HA tag||MG51561-CY|
|Mouse SCG3 Gene cDNA clone plasmid||MG51561-G|
|Mouse SCG3 ORF mammalian expression plasmid, N-Flag tag||MG51561-NF|
|Mouse SCG3 ORF mammalian expression plasmid, N-His tag||MG51561-NH|
|Mouse SCG3 ORF mammalian expression plasmid, N-Myc tag||MG51561-NM|
|Mouse SCG3 ORF mammalian expression plasmid, N-HA tag||MG51561-NY|
|Mouse SCG3 natural ORF mammalian expression plasmid||MG51561-UT|
|Learn more about expression Vectors|
SCG3, also known as secretogranin 3, is a member of the chromogranin/secretogranin family. Members of this family may serve as precursors for biologically active peptides. SCG3 is transported to secretory granules (SGs) in neuroendocrine cells. SCG3 binds strongly to chromogranin A (CgA) in an intragranular milieu and targets CgA to SGs in pituitary and pancreatic endocrine cells. With a sucrose density gradient of rat insulinoma-derived INS-1 cell homogenates, SgIII is localized to the SG fraction and is fractionated to the SG membrane (SGM) despite lacking the transmembrane region.