|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SERPINB3C ORF mammalian expression plasmid, C-GFPSpark tag||MG50625-ACG|
|Mouse SERPINB3C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50625-ACR|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-GFPSpark tag||MG50625-ANG|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50625-ANR|
|Mouse SERPINB3C ORF mammalian expression plasmid, C-Flag tag||MG50625-CF|
|Mouse SERPINB3C ORF mammalian expression plasmid, C-His tag||MG50625-CH|
|Mouse SERPINB3C ORF mammalian expression plasmid, C-Myc tag||MG50625-CM|
|Mouse SERPINB3C ORF mammalian expression plasmid, C-HA tag||MG50625-CY|
|Mouse SERPINB3C Gene cDNA clone plasmid||MG50625-M|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-Flag tag||MG50625-NF|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-His tag||MG50625-NH|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-Myc tag||MG50625-NM|
|Mouse SERPINB3C ORF mammalian expression plasmid, N-HA tag||MG50625-NY|
|Mouse SERPINB3C natural ORF mammalian expression plasmid||MG50625-UT|
|Learn more about expression Vectors|
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors). Over 1000 serpins have been identified.
Mouse SerpinB3, also known as Squamous cell carcinoma antigen 1, SCCA-1, SERPINB3, SCCA and SCCA1, is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. SerpinB3 may act as a protease inhibitor to modulate the host immune response against tumor cells. Mouse SerpinB3a and SerpinB3b, but not Serpinb3c, are functional, inhibiting both serine and cysteine proteinases with different inhibitory profiles due to the difference of two amino acids in their reactive site loops. SerpinB3a is ubiquitously expressed in most tissues, whereas expression of SerpinB3b is limited to keratinocytes. SerpinB3a and SerpinB3b may play different roles by inhibiting intrinsic or extrinsic proteinases with different expression distributions and different inhibitory profiles.