|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MET ORF mammalian expression plasmid, C-GFPSpark tag||MG50622-ACG|
|Mouse MET ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50622-ACR|
|Mouse MET ORF mammalian expression plasmid, C-Flag tag||MG50622-CF|
|Mouse MET ORF mammalian expression plasmid, C-His tag||MG50622-CH|
|Mouse MET ORF mammalian expression plasmid, C-Myc tag||MG50622-CM|
|Mouse MET ORF mammalian expression plasmid, C-HA tag||MG50622-CY|
|Mouse MET Gene cDNA clone plasmid||MG50622-M|
|Mouse MET ORF mammalian expression plasmid, N-Flag tag||MG50622-NF|
|Mouse MET ORF mammalian expression plasmid, N-His tag||MG50622-NH|
|Mouse MET ORF mammalian expression plasmid, N-Myc tag||MG50622-NM|
|Mouse MET ORF mammalian expression plasmid, N-HA tag||MG50622-NY|
|Mouse MET natural ORF mammalian expression plasmid||MG50622-UT|
|Learn more about expression Vectors|
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.