|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse DCN ORF mammalian expression plasmid, C-GFPSpark tag||MG51501-ACG|
|Mouse DCN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51501-ACR|
|Mouse DCN ORF mammalian expression plasmid, C-Flag tag||MG51501-CF|
|Mouse DCN ORF mammalian expression plasmid, C-His tag||MG51501-CH|
|Mouse DCN ORF mammalian expression plasmid, C-Myc tag||MG51501-CM|
|Mouse DCN ORF mammalian expression plasmid, C-HA tag||MG51501-CY|
|Mouse DCN ORF mammalian expression plasmid, N-Flag tag||MG51501-NF|
|Mouse DCN ORF mammalian expression plasmid, N-His tag||MG51501-NH|
|Mouse DCN ORF mammalian expression plasmid, N-Myc tag||MG51501-NM|
|Mouse DCN ORF mammalian expression plasmid, N-HA tag||MG51501-NY|
|Mouse DCN Gene cDNA clone plasmid||MG51501-U|
|Mouse DCN natural ORF mammalian expression plasmid||MG51501-UT|
|Learn more about expression Vectors|
Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.