|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PTGDS ORF mammalian expression plasmid, C-GFPSpark tag||MG51472-ACG|
|Mouse PTGDS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51472-ACR|
|Mouse PTGDS ORF mammalian expression plasmid, C-Flag tag||MG51472-CF|
|Mouse PTGDS ORF mammalian expression plasmid, C-His tag||MG51472-CH|
|Mouse PTGDS ORF mammalian expression plasmid, C-Myc tag||MG51472-CM|
|Mouse PTGDS ORF mammalian expression plasmid, C-HA tag||MG51472-CY|
|Mouse PTGDS Gene cDNA clone plasmid||MG51472-G|
|Mouse PTGDS ORF mammalian expression plasmid, N-Flag tag||MG51472-NF|
|Mouse PTGDS ORF mammalian expression plasmid, N-His tag||MG51472-NH|
|Mouse PTGDS ORF mammalian expression plasmid, N-Myc tag||MG51472-NM|
|Mouse PTGDS ORF mammalian expression plasmid, N-HA tag||MG51472-NY|
|Mouse PTGDS natural ORF mammalian expression plasmid||MG51472-UT|
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PTGDS, also known as L-PGDS, belongs to the calycin superfamily，lipocalin family. Lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in smooth muscle contraction/relaxation and a variety of central nervous system functions. PTGDS may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.