|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CLEC12A ORF mammalian expression plasmid, C-GFPSpark tag||MG50558-ACG|
|Mouse CLEC12A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50558-ACR|
|Mouse CLEC12A ORF mammalian expression plasmid, C-Flag tag||MG50558-CF|
|Mouse CLEC12A ORF mammalian expression plasmid, C-His tag||MG50558-CH|
|Mouse CLEC12A ORF mammalian expression plasmid, C-Myc tag||MG50558-CM|
|Mouse CLEC12A ORF mammalian expression plasmid, C-HA tag||MG50558-CY|
|Mouse CLEC12A Gene cDNA clone plasmid||MG50558-M|
|Mouse CLEC12A ORF mammalian expression plasmid, N-Flag tag||MG50558-NF|
|Mouse CLEC12A ORF mammalian expression plasmid, N-His tag||MG50558-NH|
|Mouse CLEC12A ORF mammalian expression plasmid, N-Myc tag||MG50558-NM|
|Mouse CLEC12A ORF mammalian expression plasmid, N-HA tag||MG50558-NY|
|Mouse CLEC12A natural ORF mammalian expression plasmid||MG50558-UT|
|Learn more about expression Vectors|
CLEC12A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. CLEC12A is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. C-type lectins are the most diverse and prevalent lectin family in immunity. Using a novel CLEC12A -specific monoclonal antibody, experiments had shown that human CLEC12A was expressed primarily on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. Although CLEC12A was highly N-glycosylated in primary cells, the level of glycosylation was found to vary between cell types. CLEC12A surface expression was down-regulated during inflammatory/activation conditions in vitro, as well as during an in vivo model of acute inflammation. This suggests that CLEC12A may be involved in the control of myeloid cell activation during inflammation.