|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human TPPP3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14360-ACG|
|Human TPPP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14360-ACR|
|Human TPPP3 ORF mammalian expression plasmid, N-GFPSpark tag||HG14360-ANG|
|Human TPPP3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14360-ANR|
|Human TPPP3 ORF mammalian expression plasmid, C-Flag tag||HG14360-CF|
|Human TPPP3 ORF mammalian expression plasmid, C-His tag||HG14360-CH|
|Human TPPP3 ORF mammalian expression plasmid, C-Myc tag||HG14360-CM|
|Human TPPP3 ORF mammalian expression plasmid, C-HA tag||HG14360-CY|
|Human TPPP3 Gene cDNA clone plasmid||HG14360-G|
|Human TPPP3 ORF mammalian expression plasmid, N-Flag tag||HG14360-NF|
|Human TPPP3 ORF mammalian expression plasmid, N-His tag||HG14360-NH|
|Human TPPP3 ORF mammalian expression plasmid, N-Myc tag||HG14360-NM|
|Human TPPP3 ORF mammalian expression plasmid, N-HA tag||HG14360-NY|
|Human TPPP3 natural ORF mammalian expression plasmid||HG14360-UT|
|Learn more about expression Vectors|
TPPP3, a member of the Tubulin polymerization-promoting protein family, is an intrinsically unstructured protein that induces tubulin polymerization. TPPP3 is a marker in the developing musculoskeletal system. In tendons, Tppp3 is expressed in cells at the circumference of the developing tendons, likely the progenitors of connective tissues that surround tendons: the tendon sheath, epitenon, and paratenon. Tppp3 is also expressed in forming synovial joints. The onset of Tppp3 expression in joints coincides with cavitation, representing a molecular marker that can be used to indicate this stage in joint transition in joint differentiation. In late embryonic stages, Tppp3 expression highlights other demarcation lines that surround differentiating tissues in the forelimb.
Depletion of TPPP3 by microRNA-based RNA interference (RNAi) inhibits cell growth, arrests cell cycles, and causes mitotic abnormalities in HeLa cells. C57BL/6 mice that received subcutaneously injected LLC (Lewis lung carcinoma) cells in which TPPP3 was knocked down showed a pronounced reduction in tumor progression. The migration/invasion activity of TPPP3-knockdown LLC cells was significantly suppressed in a transwell chamber migration assay. When these cells were injected into the tail veins of C57BL/6 mice, they exhibited milder lung metastasis compared with control tumor cells. Taken together, these findings suggested that the TPPP3 gene played an important role in tumorigenesis and metastasis, and it could potentially become a novel target for cancer therapy.